Competitive enzyme immunoassay for detection of complement-fixing antibodies in diagnostic virology.

New method for detecting Epstein-Barr virus association in nonproducer lymphoblastoid cell lines.

Epstein-Barr virus association in nonproducer human lymphoblastoid cell lines can be demonstrated by the presence of the virus genome (nucleic acid hybridization studies) or by the detection of the virus-coded complement-fixing antigen (complement fixation and/or anti-complement immunofluorescent test). This paper describes an enzyme immunoassay for the detection of Epstein-Barr virus complemen...

APPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES

Measles is one of the most contagious human diseases. Although mass vaccination programs have reduced the incidence of this disease, measles is still an important cause of morbidity and mortality among children in developing countries. Therefore the use of sensitive techniques to evaluate vaccine efficacy and level of immunity among members of susceptible communities is crucial. Serum neut...

Complement-fixing antibodies in cattle experimentally infected with Theileria annulata or vaccinated with tissue cultures

COVID-19; History, Taxonomy, and Diagnostic Molecular and Immunological Techniques

In recent years, viral epidemics such as the coronavirus disease (COVID-19) have spread, and this outbreak is thought to be the result of animal-to-human transmission. Hence, accurate diagnostic tests to detect COVID-19 and antiviral antibodies in infected individuals are of utmost importance. This report describes the structure, history, taxonomy, and molecular and immunological techniques for...

Development of a Western Blot Assay for Detection of Antibodies against HSV Using Purified HSV Virions Prepared by Sucrose Density Gradient

Objective(s) Herpes simplex viruses (HSVs) have widespread and ubiquitous prevalence in the human population and they have received a great deal of attention due to the range of diseases, they caused as a result of an infection. It seems that the fast and reliable diagnostic methods are needed for detecting the herpes simplex virus type 1 (HSV1) antibodies especially in patients with HSV encep...